With this Peptide Based Mouth Rinse Andrea has healed a cavity in between two upper teeth. Here is her story and the science.

Benefits of OraltidePro™ include:

OraltidePRO™ is a mouthwash containing DRP® (Dental Remineralization Peptide- Amorphus Calcium Phosphate) and AGDP® (Anti-gingival Degenerate Peptide).

• Promotes growth of shrinking gums
• Speeds healing of mouth & tongue
• Prevents oral infections (such as gingivitis)
• Helps with enamel remineralization
• Reduces bacteria growth and etching
• Fills slots in damaged enamel

DRP® and AGDP® Peptides

DRP® is obtained using a patented hydrolysis technology that yields a high concentration of the DRP® fragments which is then purified via ultrafiltration membrane technology. A highly negative change of the three serine phosphorylation structures chelates calcium ions to form the soluble DRP® calcium complex. This helps to contribute to the tooth enamel absorption of the calcium ion and also promotes enamel remineralization. The calcium ion leaves deposits on the damaged enamel, filling in slots, reducing bacterial growth, and preventing etching.

AGDP® is also obtained using the same patented process as DRP®. AGDP® increases synthesis of the type 1 collagen which promotes growth of shrinking gums, accelerates the healing of mouth and tongue wounds and activates gingival connective tissues and periodontal ligament fibroblasts (the cells that are thought to be responsible for maintaining the tissues.) Additionally, it promotes extracellular matrix (ECM), (molecules that are secreted by cells that provide structure and biochemical support to the surrounding cells).

Dental Bond Peptide-Amorphus Calcium Phosphate (DRP®-ACP)

• DRP® will form the DRP®-ACP complex with calcium and phosphate ion in the weak alkaline environment.
• DRP®-ACP will be adsorbed to the enamel surface to form DRP®-ACP biofilm to modulate the balance of demineralization / remineralization.
• Inhibition of calcium phosphate precipitation and crystallization, and promote remineralization reaction.
• Reduces the bacterial growth.
• Repairs damaged enamel.

Anti-Gingival Recession Peptide (AGDP®) Characteristics

• AGDP® is a small peptide which can promote fibroblast proliferation collagen and extracellular matrix (ECM), simultaneously supplement the necessary nutrients and activate the growth factors.
• AGDP® can increase synthesis of type I collagen and extracellular matrix, repairs the fracture and breakage of collagen organization, promote the growth of shrinking gums, accelerate the mouth, tongue wound healing, relieve symptoms of inflammation of the gums by activating gingival connective tissue and periodontal ligament fibroblasts.

Scientific Studies of AGDP®

Study I：AGDP® stimulated the gene expression of TGF-β.

Fig 3. (A) The TGF-β concentration of conditioned medium of tendon cells treated with AGDP® increased dose dependently. (B) AGDP® stimulates the expression of TGF-β at the transcriptional level. The resultant DNA band of TGF-β was identified at the 161-bp position.

The TGF-β concentration of conditioned medium of tendon cells treated with AGDP® increased dose dependently (157.2±4.5 pg/mL for the control group, and 164.7±6.0 pg/mL, 212.7±2.8 pg/mL, and 240.4±17.4 pg/mL for the cells treated with 0.02, 0.2, and 2.0 μg/mL of AGDP®, respectively (Fig.3A). RT-PCR of TGF-β also revealed gradual increase in TGF-β expression at the mRNA level as a function of AGDP® concentration (Fig.3B).

Scientific Studies of AGDP®

Study II：AGDP® stimulated the biosynthesis of collagen and fibronectin.

Fig. 4. Extracellular matrix stimulatory action of AGDP® occurs in a wide variety of cells and is independent of the state of cellular confluency.

The effect of AGDP® on the production of extracellularly released procollagen (A) and fibronectin (B) was quantitated. Human lung fibroblasts HFL-1 (12th passage), IMR-90 fibroblasts (12th passage), human foreskin dermal fibroblasts from normal infant foreskin (2nd passage), chick embryo fibroblasts SL-29 (15th passage), rat dermal fibroblasts (12th passage), and rat liver lipocytes (Ito cells, 6th passage) were grown in DMEM with 10% fetal bovine serum. Parallel cultures of subconfluent (■) and confluent (■) fibroblasts were treated with 50 μM AGDP®. Quantitation of extracellularly released radiolabeled procollagen and fibronectin was accomplished and data are shown as mean ± S.E. derived from three independent experiments. The result is indicated that AGDP® can stimulate production of matrix molecules such as collagen and fibronectin from several fibroblast cell lines of human, mouse, rat, and chicken origin.

Scientific Studies of AGDP®

Study III：AGDP® synergized with TGFβ1 up-regulate ECM biosynthesis.

Fig 5. Augmentation of TGFβ1-induced ECM biosynthesis by AGDP®. Subconfluent HFL-1 cells washed with phosphate-buffered saline were preincubated for 24 h in DMEM containing 0.2% serum and treated with TGFβl (0, 0.5, and 5 ng/ml) in the absence (solid lines) or presence (broken lines) of 50 μM AGDP® for an additional 24 h. Radiolabeled polypeptides released in the medium were analyzed as previously described. The effect on procollagen (■) or fibronectin (■) production is shown, and results are expressed to depict-fold stimulation as treated/control with mean ± S.E. calculated from three independent experiments.

To examine if AGDP® synergized with TGFβ1, a well known stimulator of extracellular matrix biosynthesis, we treated the six cell lines either with TGFβ1 alone (0.5 or 5 ng/ml) or simultaneously with AGDP® (50 μM). TGFβ1 stimulated ECM production in both confluent and subconfluent cells and was further augmented by AGDP® in subconfluencte lls (Fig. 5), suggesting that TGFβ1 and AGDP® up-regulate ECM biosynthesis by independent mechanisms.

Scientific Studies of AGDP®

Study IV：High concentration of AGDP® regain the CAL

Radiographic outcome of a FGF-2-administered individual. A 6

% AGDP-administered 39-year-old woman. The arrows indicate the remaining alveolar bone crest or the bottom of the bone defect. The depth of the intraosseous defect before administration was measured at 3.0 mm on the x-ray. The radiographs clearly show that the bone defect was filled with the newly generated alveolar bone at 36 wks after administration. The percentage of bone fill at 36 wks was 69.14%, with 3 mm CAL regained.

OraltidePro™

The high concentration of edible DRP® and AGDP® peptides simultaneously repair damaged enamel, treat gingival recession and anti-inflammatory effect, and add 5% xylitol antibacterial effect, the overall effect is doubled and comprehensive while relieving sensitive teeth.

Products are made of whole food grade raw materials, no addition of alcohol, fluoride, anti-sensitive potassium nitrate, and strontium chloride, bactericides Is a comprehensive dental care products.

Protection of dentin

Increase dentinal tubules anti-hypersensitivity by using DRP®-calcium complex/AGDP®.

Refill micro aperture

Calcium ion deposits on the damaged enamel to reminersalize, fill the slot, reduces bacterial growth and prevent etching.

Repairs periodontal connective tissue

Promoting collagen and extracellular matrix (ECM) synthesis, activating growth factors to accelerate the mouth, tongue wound healing.

Repairs gingival recession

Promote the growth of shrinking gums, relieve symptoms of inflammation of the gums.

Overall oral health improvement

Repair damaged enamel, anti-hypersensitivity, strong gums healthy, reduce the rate of growth of bacteria, solve periodontal problems from the root cause.

OraltidePro™ Customer reviews

Please see attached for the before and after photos from the Invisalign app. The date shows the date when I started Invisalign, but I have only applied OraltidePro regularly for the last few months or so. The chipped tooth is in the middle on the bottom. Note the tooth now looks better when viewed from above in real life - the photos are somehow from below. It has also further improved significantly in the last month since the after photo